Adsorbout treatment protocol https://www.ashi-hla.org/forums/posts.aspx?topic=1460058 Sat, 6 Jun 2026 11:44:45 GMT Fri, 9 Nov 2018 16:12:10 GMT Copyright © 2018 American Society for Histocompatibility and Immunogenetics Adsorbout treatment protocol https://www.ashi-hla.org/forums/posts.aspx?topic=1460058 https://www.ashi-hla.org/forums/posts.aspx?topic=1460058 We are considering treating all Single Antigen bead assay sample aliquots with Adsorbout at the start, instead of reflexing if the NC is high. Our protocol for treating serum is to treat either 40 or 80 uL of serum with Adsorbout, incubating in a round-bottom 96-well tray, then pipette into new tubes to spin down Adsorbout beads. It's cumbersome and so techs aren't thrilled with the idea of treating all samples.

What type of tray or tube do others use for Adsorbout treatments?

Does anyone treat in PCR-sized tubes or 0.7mL tubes? I'm concerned about getting a good rock/rotation with small volume.

Our first test will be treating 60uL serum in 0.7mL tubes, laying tubes on the sides, incubate on rocker. This would minimize the number of times we move sample to a new tube, but will the sample get adequately Adsorbed? We shall see...


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