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ASHI Governance
Standards for Histocompatibility Testing Section M - Disease Association M1.000
Complete HLA typing is an appropriate option. M1.100
Typing may also be limited to all products of a single or limited
number of HLA loci. M2.000
Typing for a single antigen (e.g., HLA-B27). M2.100
Cell controls must be tested on each batch of typing-trays. M2.110
The control cells must include at least two cells known to express the
specified antigen. M2.120
The control cells must also include two cells for each crossreacting
antigen which might be confused with the specific antigen. M2.130
The control cells must also include at least two cells lacking the
specific and crossreacting antigens. M2.200
Serum controls must be tested at the time of typing. M2.210
Serum controls must include a positive and negative control. M2.220
Serum controls should also include two sera for each antigen which
crossreacts with the specified antigen (if available). M2.300 Sera to define each antigen must meet requirements of Sections E or F as appropriate. |
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