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ASHI Governance
Standards for Histocompatibility Testing Section D - HLA Antigens D1.000
Terminology of HLA antigens must conform to the latest report of the
W.H.O. Committee on Nomenclature. D1.100
Potential new antigens not yet approved by the W.H.O. Committee must
have a local designation which cannot be confused with W.H.O.
terminology. D1.200
Phenotypes and genotypes should be expressed as recommended by the
W.H.O. Committee, as in the following examples: D1.210
Single antigens: HLA-B7 (or B7 if HLA is obvious from context). D1.211
The locus designation must always be included. D1.220
Phenotype: HLA-A2,30; B7(Bw6), 44(Bw4); Cw5; DR1,4; DQ5,7; Dw1,w4. D1.221
If only a single antigen is found at a locus, the phenotype may
include it twice only if homozygosity is proven by family studies.
Conversely, a “blank antigen” can only be assigned if proven by
family studies. D1.230
Genotype: HLA-A2,B44(Bw4),Cw5,DR1,DQ5,Dw1/A30,B7(Bw6),
Cwx,DR4,DQ7,Dw4. D2.000
Determination of haplotypes and genotypes can only be done by family
studies. D2.100
Family studies. D2.110
All available members of the immediate family should be typed. D2.111
Typing for HLA-A,-B locus antigens is mandatory. Typing for HLA DR is
highly recommended. D2.112
Typing for HLA-C, -D, -DQ and/or -DP may be helpful in some situations
but is not mandatory. D2.113
Reports of HLA family studies must include haplotype assignments and
an explanation of recombination when this occurs. D2.200
Unrelated individuals. D2.210
The probability of possible haplotypes, given the phenotype, may be
determined from known haplotype frequencies in the relevant
population. D2.220
The haplotype frequencies used should be from the most complete and
reliable studies available. D2.230
The haplotype frequencies used should be those most appropriate for
the ethnic group of the subject. D2.240
Reports of probable haplotypes based on population frequencies should
clearly indicate that they were so derived. D3.000 The laboratory must have a written policy that it follows that establishes when antigen redefinition and retyping are required. |
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