This is an archive from the old ASHI Forum

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Padma

Posted: Fri Feb 06, 2009 2:39 pm
Why would one see false negative amplification while all internal control bands worked? What are possible interferences that would not amplify positive bands.

Marker worked fine. DNA conc 135 ng/ul and ratio of 1.96

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cavergas

Posted: Mon Mar 09, 2009 10:25 am
Were there any positive bands at all? Also, how strong were the internal control bands? Could be a novel allele that the primer(s) are not annealing to. Not sure of answer without having more detail.