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alzayerfadi

Posted: Sat Nov 25, 2006 8:17 am Post subject: AHG-CONTROL Reply with quote
I would like to know, how you QC the AHG for the CDC crossmatch?
Any ideas..
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Fadi Al Zayer. CHT.CHS(ABHI)

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mpdowns

Posted: Thu Nov 30, 2006 11:42 am
Some ideas include:
Titer the control against the current AHG dilution, as well as using some wells for no complement, and/or inactivated complement , and parallel this with same set-up and no-AHG. This way you know if it is toxic on its own, what the endpoint is, and if it actually works. We have found that it is not consistent with all cells/targets depending on where you get your AHG control.
You could also run it on a cell panel tray at different dilutions with and without AHG.