This is an archive from the old ASHI Forum

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William W. Ward

Posted: Thu Aug 17, 2006 4:33 pm
I am going to be drafting a section for a new CLSI (formerly NCCLS) document on “HLA Detection by Flow Cytometry” dealing with special considerations for antibody testing using the microparticle solid phase assays on the flow and Luminex platforms.

I would like to hear from anyone with technical pointers to share on the interpretation of these assays, especially how to identify and avoid “pitfalls”. Some considerations along these lines might include: Proper selection and use of negative controls; How to handle “Left-Shift” results; What to do about “Anti-bead” antibodies and other causes of high background; Just How Do You Define a Positive result? Is a weak result relevant, and if so, how weak? etc.

Any and all suggestions for topics to include in this section are welcome. Please email me directly if you don’t wish to respond here.

Thanks - Bill Ward

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shelman

Posted: Mon Jun 09, 2008 10:18 am
Was this ever completed?

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William W. Ward

Posted: Fri Jul 11, 2008 1:14 pm
Yes. The project was completed and published as a proposed guideline. It has been through the review process and I believe it is now an approved guideline. I haven’t seen it advertised for sale yet though.