Hi, we would like to do a quick survey on your HLA genotyping experience and preferences to draw a picture of the field. Please take few minutes of your time to fill in the following form. You can send it back to my email address or FAX it to: (514)904-8559. Thanks a lot! Lucie

HLA Center and Affiliation:
Name of respondent or lab director:
• How many sample analysed per year:
• Nature of the sample:
o Blood:
o Buccal swabs:
o FTA papers:
o Other (please precise):
• rSSO (low res) main vendor:
o Failure rate* observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
(*: The failure rate is defined by the impossibility to give a result and the need to repeat the analysis.)
• rSSO (low res) other vendor used:
o Is failure rate lower, similar or higher as compared to your main vendor:
• SSP (low res) main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
• SSP (low res) other vendor:
o Is failure rate lower, similar or higher as compared to your main vendor:
• SSP (high res) main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1: 

• SSP (high res) other vendor:
o Is failure rate lower, similar or higher as compared to your main vendor:
• SBT double strand main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
• SBT double strand other vendor used:
o Is failure rate lower, similar or higher as compared to your main vendor:
• How many complementary sequences (ex: HARPS) done per locus (average):
• SBT single strand or new generation main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
• Do the observed failure rates obtained from your main vendor’s kits meet your goals:
• Do the failure rates that you observed remain constant from year to year or do they vary:
• Other typing techniques used:

• Type of DNA extraction system and vendor:
• Whole genome amplification:
• Comment: