Our lab is looking for advice on resolving the two CWD alleles B*4402/4441 for bone marrow high-resolution typing.
Using our standard methods of One Lambda HD-SSO, sometimes these alleles can’t be resolved, especially in a patient who is also either B*15 or B*40.
My question: when would you rule out B*4441 by allele frequency?
1) Both alleles are CWD alleles (Cano et al. 2007), and this is our current standard for HR results.
2) The NMDP allele frequency tables show that 4402 is very common in the US (high frequency and rank), 4441 is not listed. However, being new to HR typing, we are nervous about using this data over the CWD standards.
3) Allele frequencies.net shows that B*4441 has the allele frequency of 0.000 for the US.
4) Literature searches and HistoCheck show that B*4441 differs from B*4402 by 2 nucleotides and 2 amino acids.
Thank you for any advice or discussion!
