This is an archive from the old ASHI Forum

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dannyy

Posted: Thu Feb 14, 2008 3:27 pm Post subject: B-cell Antiglobulin Crossmatch Reply with quote
Many years ago our lab played around with using AHG in the B-Cell cytotoxicity crossmatch, with mixed results. Some cells seemed to be overly susceptible to the reagent and had high cell-death backgrounds in all sera, while others did just fine. We’re planning to evaluate B-cell-AHG again, and would apprecaite hearing others’ experience and advice.

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awhiteley

Posted: Mon Dec 01, 2008 1:46 pm
I think the main issue with B-Cell crossmatching is the CD32 receptors and the non-specific binding of IgG by the FcgR. Does your lab already do a B-cell Flow Crossmatch?

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dannyy

Posted: Wed Jan 21, 2009 1:47 pm
Yes, we perform a B-cell Flow XM, but there are some applications where our customers prefer a cytotoxicity crossmatch.

Since many B cells have Ig on their surface, the anti-Ig will react. Most B cells have IgM and IgD, but some also have IgG. The magnitude of the ‘background’ killing you will get depends on the anti-globulin reagent that is used. If you use an antibody to Ig light chains, as is common in the T-cell AHG test, you may get a lot of B cell killed. We prefer not to do this test.

Good luck!

-PS