This is an archive from the old ASHI Forum

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cjordan

Posted: Thu Sep 14, 2006 4:30 pm Post subject: GTI EZ Type SSP System Reply with quote
Is anybody out there in HLA land using the GTI EZ Type SSP System?
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Curt Jordan
Carter BloodCare/Stewart Regional Blood Center
Tyler,TX

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vicki22

Posted: Wed Oct 04, 2006 12:55 pm
We have started to qualify the DRDQ kits but they are not in use yet. We just wanted a backup for our current Pel-Freez SSP trays so we are not in a hurry to implement.

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cjordan

Posted: Wed Oct 04, 2006 3:03 pm
Thanks!

Let me know how you like them. We were having some problems with the ABC trays. getting alot of no amps. I’m just trying to see how many people are using the trays, and if having some of the same problems Smile

The DR/DQ trays seem to be OK
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Curt Jordan
Carter BloodCare/Stewart Regional Blood Center
Tyler,TX

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jtownsend

Posted: Mon Feb 02, 2009 4:14 pm
We use the ABC and DRDQ routinely for deceased donor typings. It is a good system for going molecular for this type of work. Easy to get up and running. A few issues: paperwork a little confusing to start with, and software needed updates but it has been and we will see it in March. Some very weak bands are false positive because they have the correct bp size. Very low resolution, but good for serological equivalence and sure beats serology. Don’t recommend using the heat sealer, can melt trays to the point of not being able to insert pipet tips into wells to extract amplicons, ok just to use adhesive seal.

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cjordan

Posted: Tue Feb 03, 2009 11:56 am
agreed, be careful with the heat sealer, no more than about 5-6 seconds. we have melted trays also. the paperwork and analysis is a little more complicated than other kits.
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Curt Jordan
Carter BloodCare/Stewart Regional Blood Center
Tyler,TX