ASHI Forums

Survey on genotyping experience and preferences

2011-09-16T10:53:09-05:00

Hi, we would like to do a quick survey on your HLA genotyping experience and preferences to draw a picture of the field. Please take few minutes of your time to fill in the following form. You can send it back to my email address lucie dot richard at hema-quebec dot qc dot ca or FAX it to: (514)904-8559. Thanks a lot! Lucie

HLA Center and Affiliation:
Name of respondent or lab director:
• How many sample analysed per year:
• Nature of the sample:
o Blood:
o Buccal swabs:
o FTA papers:
o Other (please precise):
• rSSO (low res) main vendor:
o Failure rate* observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
(*: The failure rate is defined by the impossibility to give a result and the need to repeat the analysis.)
• rSSO (low res) other vendor used:
o Is failure rate lower, similar or higher as compared to your main vendor:
• SSP (low res) main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
• SSP (low res) other vendor:
o Is failure rate lower, similar or higher as compared to your main vendor:
• SSP (high res) main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:

• SSP (high res) other vendor:
o Is failure rate lower, similar or higher as compared to your main vendor:
• SBT double strand main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
• SBT double strand other vendor used:
o Is failure rate lower, similar or higher as compared to your main vendor:
• How many complementary sequences (ex: HARPS) done per locus (average):
• SBT single strand or new generation main vendor:
o Failure rate observed for each locus tested:
 A:
 B:
 C:
 DRB1:
 DQB1:
• Do the observed failure rates obtained from your main vendor’s kits meet your goals:
• Do the failure rates that you observed remain constant from year to year or do they vary:
• Other typing techniques used:

• Type of DNA extraction system and vendor:
• Whole genome amplification:
• Comment:

B*4402 vs 4441

2009-10-15T17:40:29-05:00

Our lab is looking for advice on resolving the two CWD alleles B*4402/4441 for bone marrow high-resolution typing.
Using our standard methods of One Lambda HD-SSO, sometimes these alleles can’t be resolved, especially in a patient who is also either B*15 or B*40.

My question: when would you rule out B*4441 by allele frequency?

1) Both alleles are CWD alleles (Cano et al. 2007), and this is our current standard for HR results.
2) The NMDP allele frequency tables show that 4402 is very common in the US (high frequency and rank), 4441 is not listed. However, being new to HR typing, we are nervous about using this data over the CWD standards.
3) Allele frequencies.net shows that B*4441 has the allele frequency of 0.000 for the US.
4) Literature searches and HistoCheck show that B*4441 differs from B*4402 by 2 nucleotides and 2 amino acids.

Thank you for any advice or discussion!

Bone Marrow Transplantation Histocompatibility forum?

2009-08-18T13:08:28-05:00

Is there a web forum out there that focuses on bone marrow transplantation histocompatibility, through NMDP, ASHI, or others?

Since our lab is now offering high-resolution DNA typing to support our university’s program, it would be great to connect with similar labs. Possible topics: reporting NMDP codes and CWD alleles, high-resolution typing techniques such as HD-SSO technology, and how/when to report allele groups, etc.

I’d appreciate forum responses or personal e-mails.
Thanks~
Carley

Greetings from Dallas

2009-07-03T11:40:46-05:00

We used to have a ‘Digital Discussions’ forum that was quite active. I wonder if we can get it started again. Does this Bulletin Board notify people by e-mail when there are new messages that might be of interest to them? Perhaps it is available and needs to be turned on… Lets hear from you!

-Peter