IMMUNOMODULATORY EFFECT OF BLOOD TRANSFUSION WITH DIFFERENT TIME
OF STORAGE
K. Dimitrovski, V. Zdravkovska, P. Kolevski. Institute of blood
transfusion, Skopje, Rep. of Macedonia.
The shortage of blood in our institution
forced us to spend it in period of one week after donation. We choose this
period to investigate immunomodulatory effect of blood for transfusions.
METHODS: We have investigated 49 dialysis patients. The immunomodulatory
effects were investigated with: Screening of HLA antibodies with cross-match;
Mixed lymphocyte culture (MLC), using donor MNC as stimulators and identical
positive control; Lymphocyte transformation test (LTT). All tests were
done before and 15-20 day after the transfusion.
RESULTS: Antibodies after the transfusion were detected at 14 patients.
10 of them received blood stored less than 4 days, and the rest got blood
stored 4-7 days (p<0.05, hi2= 3.997). The results of proliferative tests
(MLC, LTT) are presented as sum of the differences between first and second
sample at each patient.
Table 1. Differences in the reactivity in proliferative tests from
the first sample
| < 4 days |
dMLC |
dPHA |
dConA |
dPrA |
Dpwm |
| (N=25) |
109.09% |
113.09% |
103.49% |
108.55% |
104.67% |
| >4 days |
88.87% |
106.56% |
106.15% |
102.29% |
111.23% |
| (N=24) |
3.26 |
0.97 |
0.44 |
0.53 |
0.77 |
| |
S |
NS |
NS |
NS |
NS |
Secondary MLC show significant difference if patient
received transfusion of blood stored less or more than 4 days. The fresher
blood shows increment of the response in secondary MLC, but LTT does not
show such differences.
CONCLUSION: The storage time is one of the factors that should be taken
on mind in the process of blood delivering before transfusion at patients
with different diagnosis.