THE SHARED EPITOPE INFLUENCES THE EXTENT AND PROGRESSION OF RHEUMATOID ARTHRITIS.
          A. Valenzuela, M.F. González-Escribano, R. Rodriguez, K. Walter, I. Moreno, B. Sanchez, A. Garcia & A. Nuñez-Roldan.

          Servicios de Inmunología and Reumatologia. Hospital Universitario Virgen del Rocío. Servicio Andaluz de Salud. 41013. Sevilla. Spain. Susceptibility to rheumatoid arthritis (RA) has been classically associated with the inheritance of a shared epitope (SE) encoded by several different MHC class II alleles. This epitope encompasses the sequences LQRA, LQKA, or LRRA at positions 67, 70, 71, and 74, in HVR3 of the DRb1 first domain. In the last few years some discussion has emerged around the role of the SE in RA severity and progression, rather than in disease susceptibility. To get more insight into this question, a 8 year prospective study was performed on 82 unrelated Spanish RA patients with a disease duration of less than two years at the moment of the study entry. Fourteen patients carried two SE+ HLA-DRB1 alleles (SE +/+); 36, one single allele (SE +/-) and 32 were SE negative (SE -/-). Radiological evaluation of disease severity was performed at the time of diagnosis and 5 and 8 years later at study entry, the frequencies of locally severe (hands and feet) RA were: 79%, 44% and 44% among SE +/+, SE +/- and SE -/- patients respectively (p=0.05; RR=1.8; 95% CI: 1.11-2.9 and p=0.05; RR=1.78; 95% CI: 1.12-2.8, respectively). As disease progresses, these differences disappear whereas differences in the extent of the disease arise: after 8-10 years of disease outcome, 79%, 49% and 32% of SE +/+, SE +/- and SE -/- patients respectively showed large joints involvement (shoulders, elbows, hips and knees) (p=0.01; RR=2.44; 95% CI: 1.37-4.35). These results suggest that the presence of the shared epitope is associated with the extent and progression of rheumatoid arthritis.