HLA-DQ6, TNFa11 ALLELE and HPV SEROPOSITIVITY INCREASES THE RISK SIGNIFICANTLY HIGH FOR SWEDISH WOMEN WITH CARCINOMA CERVIX.
C.B. Sanjeevi, M. Ghaderi, C.S. Peacock, P. Hjelmström, G. Hallmans, F.Wiklund, P. Lenner, J.M. Blackwell, J. Dillner.   Department of Molecular Medicine, Karolinska Hospital, Microbiology and Tumor Biology Centrum, Karolinska Institute, Stockholm, Department of Nutritional Research, University of Umeå, Umeå, Sweden; Department of Medicine, University of Cambridge, UK. 

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Carcinoma Cervix is known to be associated with HPV infection, DR15-DQ6 (DQB1*0602). The aim of the study was to analyze the association of TNFa, HPV16 and DQ6 positivity in patients and controls from the Vasterbotten county in Northern Sweden. TNFa typing was done by PCR-microsatellite typing and HLA genotyping for DQ was done by PCR-SSO in 64 patients and 147 matched unrelated controls. HPV16 antibodies were identified by ELISA using capsid protein as the antigen. TNFa 11, DQ6 and HPV16 seropositive patients are presnt in 11/64 (17%) patients and 2/147 (1%) controls (Odds ratio 15.05; 95% confidence interval = 4.49-50.4; p<0.0001). Results show that the alleles of the TNFa 11, DQ6 and HPV16 seropositivity are significantly associated in patients with Carcinoma cervix compared to controls. Individually the risk conferred by them are 2 to 3 times compared to controls, but together the risk increases to 15 times when compared to the controls. This information is valuable in prediction strategies for the disease. In conclusion, CaCx susceptibility is increased by TNFa 11 allele in DQ6 positive and HPV16 seropositive patients.