3.916667
#2
STUDY OF BRAZILIAN PATIENTS PRESENTING THE HLA-DRB1*15011 GENE AND HIGH MORTALITY RATES AFTER LIVER TRANSPLANT.
Margareth Batistella Araujo Doctor , Luis Sergio Leonardi Doctor , Marilia Iracema Leonardi Doctor , Luis Antonio Magna Doctor and Maria Helena Stangler Kraemer Doctor . 1 Department Clinical Pathology, State University of Campinas, Campinas, Sao Paulo, Brazil ; 2 Department Surgery, State University of Campinas, Campinas, Sao Paulo, Brazil and 3 Department Genetics, State Universite of Campinas, Campinas, Sao Paulo, Brazil .
Liver transplant is the definitive therapeutic modality for liver diseases in terminal stage. Some specific recipient alleles were associated with high risk of acute rejection and tolerance soon after transplant. Our objective was to verify the importance of the HLA-DRB1*15011 alleles in recipients after liver transplant. Analyses were carried out in peripheral blood of 50 recipients after liver transplant. DNA was extracted from the samples and typing of HLA-DRB1 alleles was obtained by Polymerase Chain Reaction using medium and high resolution Sequence-Specific Primers (PCR/SSP). The HLA-DRB1*15 allele was found to be present in 10 out of 50 examined patients and comparison of frequencies in healthy individuals and in patients did not result in any statistically significant differences. However, analysis of frequency carried out within the group of the 10 patients carrying the DRB1*15 allele comparing deceased patients with survivors resulted in a positive association (p=0,007, OR= 8,5). Subsequent high-level typing of HLA-DRB1 revealed 7 patients with HLA-*15011 and of those 5 (71%) died and 2 (29%) survived, which shows a highly significant association (p= 0,0001, OR= 12,85). A more detailed investigation on deceased patients carrying the HLA-DRB1*15011 allele is needed to determine the effect of the HLA on that group of patients after allogenic liver transplant as a predictive value of the individual risk for each patient.