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#59
ASSOCIATION BETWEEN HLA-B57, KIR GENES AND HIV PROGRESSION.
M. R. Uribe, BS, S. Adams, BS, W.L. Shupert, D. Stroncek, M. Connors and F. Marincola. Bethesda MD, USA, National Institutes of Health, 20892-1184, HLA Laboratory, DTM.

Association between HLA-B5701 and long term nonprogressors HIV infected patients has been reported previously. Due to the role that NK cell receptors and HLA molecules play in the immune response against viral infections, KIR genotyping was performed to determine, if there exist an additional association.
Sixteen DNA samples, predetermine to be B57 from HIV + pts, eleven from long term nonprogressors (LTNP), and five from progressors (P) were KIR typed by a low resolution PCR-SSP method, and HLA typed by sequence-based typing. Twelve were B5701, three B5703, and one B5701, 5703. KIR haplotype A/B was present on 8 of the LTNP. KIR 3DS1 with the corresponding HLA ligand was present in 7 of the LTNP, and 2 of the P. In this preliminary analysis no association was found between the KIR haplotypes, individual KIR genes and status of disease progression. Coincidentally we observed that 1 of 11 LTNP did not express B5701 while 2 of the 5 progressors did, not suggesting a possible role for allele variability among the two populations. However, since both due to the fact that B5701 and B5703 are ligands for KIR3DS1 both bearing an Isoleucine in position 80, it is unlikely that these differences could be of relevance in this analysis. Overall low resolution KIR typing did not demonstrate in this small series any association with disease progression. Further studies using high resolution KIR typing and evaluation of actual gene expression in larger pt cohorts should be performed in the future.

Association between HLA-B57 alleles and disease progression
HLA-BPLTNP
5701310*
570322*
* One pt was HLA-B5701,B5703



Association between KIR haplotypes and disease progression
KIR HaplotypePLTNP
A23
A/B38