4.0000
#129
THE HLA-A, B AND DRB1 POLYMORPHISM IN THREE BRAZILIAN POPULATIONS.
Leonardo M. Ferreira, Ceusa Schafhauser, MSc, Madalena Okoinski, Helena B. Cazarote, Ivy N. Vieira, Sandra M. Ferreira, Claudia H. Takimura, Juliana P. Camargo, Gisele C. Possebon, Luciane Kaminski, Vania C.F. Bandeira, Moramay Z.P. Ikeda, Cleide Santos, Taiana B. Bertozzi, Carlos F. Alves, Vanessa S. Paladino and Cristina Q.C. von Glehn, MSc. Curitiba Parana, Brazil, Cajuru University Hospital / Health Alliance / PUCPR, 80050-350, Immunogenetics Laboratory.

---

There is a genetic complexity of the HLA system both regard to the extensive polymorphism and polyallelism that differs greatly among populations.
The purpose of this work was calculated the allele frequencies for HLA-A, B and DRB1 in samples of the 3 Brazilian States: Parana (PR) (n=5131), São Paulo (SP) (n=526) and Mato Grosso (MT) (n=140). The HLA typing was done by PCR/SSP techniques. The allele frequencies were obtained by direct count. We observed for HLA-A genes 21 alleles for PR, 19 for SP and 17 for MT. A*02, A*01 and A*24 were the most frequents in the 3 populations samples. For HLA-A*30 were observed a frequency of the 7.1% for MT sample and 4.1% to PR and SP samples. For HLA-B genes were observed 35 alleles for PR, 30 for SP and 29 for MT. B*35, B*51 and B*15 were the most frequents in the 3 populations samples. Differences on frequencies for HLA-B*42 (MT=3.9%, PR and SP=1.0%), B*53 (MT=4.6%, PR1.6% and SP=2.8%) were found. For DRB1 genes were observed 13 alleles in the 3 populations, the most frequents were DRB1*13 and *04 and were observed differences on frequencies for DRB1*07 (MT=6.4%, PR=11.6% and SP=13%) and DRB1*12 (MT=3.2%, PR=1.6% and SP=0.9%).Conclusion: In this preliminary study the MT population size is very smaller than PR and SP populations. Although the differences of the population size, the different allele frequencies found show us the Brazilian population admixture is different from one state to another.