#109
GENETICALLY CONDITIONED SYNTHESIS LEVEL OF PROINFLAMMATORY CYTOKINE TNF-ALFA AND IL-6 IN A GROUP OF HLA- HAPLOIDENTICAL DONOR /RECIPIENT PAIRS OF CONJUNCTIVAL LIMBAL STEM CELLS.
Edward Wylegala, dr and Urszula Siekiera, dr. Katowice Poland, Railway District Hospital, Department of Ophtalmology and Katowice Poland, Blood Center, HLA
Immunogenetics Laboratory.
Background : Certain pathologic conditions cause limbal stem cells deficiency. Identification of immunogenetic factors influencing outcome after CLAL-lr (conjunctival limbal allograft from living related donors) was the aim of our research. HLA compatibility and genetically conditioned synthesis level of TNF-alfa and IL-6 was defined in a group of 21 donor / recipient pairs in relations : parent / child (11 pairs), child /parent ( 7 pairs) and siblings (3 pairs).
Main outcome measures after transplantation was reconstruction of the corneal surface epithelium, restoration of avascularity and improvement in visual acuity.
Material and methods : Peripheral blood lymphocytes were the source of genomic DNA. HLA -A*, B*, DRB1* alleles, TNF-alfa, IL-6 genetypes connected with their synthesis level were typed using PCR-SSP method.
Results : During the observation period (36 months ) we noticed graft rejection in 30 % ( 7/21) and outcome in 70% (14/21) of recipients. Successful transplantation was observed more frequently among donor / recipient pairs in the relation parent / child. There was no difference in the effect of conjunctival limbal stem cells transplantation observed in a group of high and low producers of TNF-alfa . IL-6 genotype GG and GC ( high producers ) carryed mainly successful graft recipients. Genotype CC (low producers) was observed mainly in fail cases.
Conclusion : HLA matching decreases rejection rensponse. In addition proinflammatory cytokine synthesis level profile seems to be the factor determining patient immune responder status plays the role in the donor / recipient tissue dialogue.