3.4
#71
HLA ASSOCIATIONS IN ANCA POSITIVE PATIENTS FROM MUMBAI, INDIA.
Shankarkumar Umapathy Ph.D , Devaraj Prasanavar M.Sc , Vandana Pradhan M.Sc and Suresh Badakere Ph.D . Mumbai Maharashtra, India, Institute of Immunohaematology (ICMR), 400012, HLA .

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Antineutrophil cytoplasmic antibodies (ANCA) are autoantibodies against enzymes present in primary granules of neutrophils and monocytes, which are mainly detected in systemic vasculitis and in other infectious diseases as well. There is considerable evidence that genetic factors influence susceptibility to ANCA associated vasculitis as well as ANCA may have a role in disease pathogenesis, since autoreactive T-cells from patients with vasculitis have been shown to proliferate invitro in response to the target antigens of ANCA proteinase 3 and myeloperoxidase. Most T- cell dependent autoimmune diseases show clear positive and/or negative HLA allele association. These HLA molecules play a central role in the generation of T-cell responses. Therefore we compared the HLA A and B loci alleles from 84 ANCA positive patients comprising of Malaria (34) Leprosy (23) and Pauci immune vasculitis (27) with ethnically same age and sex matched normal controls (101) from Mumbai. The HLA antigens were serologically identified using standard NIH technique. The results revealed that HLA A1 (pt 46% vs 18%; OR = 3.96; P value 2.72E - 05), B8 (6% vs 3%; OR = 3.43; P value 0.06), B17 (30% vs 13%; OR = 3.38; P value 0.0008) and B40 (46% vs 27%; OR = 2.74; P value 0.001) were increased significantly among the ANCA patients when compared to the controls. The study reveals that A1, B8, B17 and B40 are associated with ANCA production in these patients.