3.1
#59
AUTOMATION OF FLOW CYTOMETRIC ANTIBODY SCREENING AND REPORTING.
Stephanie Tipton MS, CHT , Kim Holden BS, CHS , John Kreuziger , Ellen Klohe Ph.D. , Scot Townshend and Susan Metz . Spokane WA, Inland Northwest Blood Center, 99201, HLA Laboratory and Chantilly VA, SystemLink, Inc., 20151 .

---

One Lambda Flow PRA™ beads are a highly sensitive and specific means to determine the presence of IgG anti-HLA antibodies in patient sera and is the method we employ for monthly antibody screening of our growing kidney transplant candidate list. We have incorporated software interfaces to accommodate the management of large volume test data generated by the One Lambda FlowPRA™ screening assays. SystemLink’s lab management software, HistoTrac™, facilitates the data management through the use of an interface with the Becton-Dickinson flow cytometer analysis software, CellQuest™, and through the ability to upload PRA results into UNet. The HistoTrac™ batch and labeling modules allow for the accessioning and sample label creation for large numbers of samples. Once a batch is created, a worklist is set up using the interface and imported directly into the Worklist Manager program for acquisition by AutoLoader™ on the FACSCalibur™ flow cytometer. Following acquisition, batch analysis of the data is performed with CellQuest™ and the PRA values generated from the analysis are exported, for import into HistoTrac™. Once reviewed, the PRA data are exported from HistoTrac™ for upload into UNet. This streamlining of data has significantly reduced the time required for data entry and reduced the potential for transcription errors. Additionally, since the interfaces occur at multiple stages, it is possible to make changes or additions throughout the process.