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#48
A RARE HLA A*02 SUBTYPE FOR WHICH THE SEROLOGICAL PATTERN IS NOT DETERMINED.
Luz A. Stamm, BSc, MLT , Charlene Ott MLT, ART , Irene Shewchuk MLT , Iwona Galaszkiewicz MLT,ART , Elizabeth Potok MLT , Leona Kowalick MLT , Jane Hickerty MLT , Marcelo F. Vina, PhD and Matin Gutierrez BSc . Calgary AB, Canada, Calgary Laboratory Services, FMC site, T2N 2T9, Tissue Typing and Kensington MD, USA, Georgetown Medical Center, 20895, Department of Oncology and Pediatrics .
It is important to characterize the serological assignment of rare alleles in research strategies for bone marrow allogeneic stem cell transplantation. Our laboratory uses serological typing as the primary technique for identifying HLA-A and B antigens in identical siblings for family studies. Homozygous typings for HLA-A and B are then confirmed by Reverse SSOP (Dynal Reli®) and/or SSP (Genovision).
An AML recipient and five siblings were typed in our laboratory. The patient and one of the siblings tested homozygous for A24. Confirmation of the HLA-A locus was performed by Reverse SSOP (Dynal). Results showed an A*02XX and A*24XX. The SSP (Genovision) results were unclear showing an A*02013 with some additional amplified PCR products. The patient sample was sent for DNA sequencing. The A2 allele in this patient was confirmed as A* 0230. Our serological typing trays (One Lambda T2-72 and GTI-72) were unable to detect this A2 allele.
Family Haplotyes A B C DR DQ Patient A*0230 A24 38,56 1 *01XX *13XX *0501 *0603 Sib #1 A*0230 A24 38,56 1 *01XX *13XX *0501 *0603 Sib #2 A*0201 A24 37,56 1 *01XX *01XX *0501 *0501 Sib #3 A*0201 A*02 37,27 2 *15XX *01XX *06XX *0501 Sib #4 A*0201 A*02 37,27 2 *15XX *01XX *06XX *0501 Sib #5 A*02 A*0230 27,38 2 *13XX *15XX *0603 *06XX
A*0230 was assigned in 1998 and has been described in three Caucasoid individuals. Interestingly, two of the three individuals have B*3801 as a common HLA-B allele. Our AML patient and sibling fall into this haplotype category.