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THE INVESTIGATION OF HLA-A,B,DR GENE FREQUENCY IN NORTHERN HAN CHINESE POPULATION.
Yiming Guo Bachelor , Changying Li Bachelor , Shuxian Jiao Master , Zhongsi Yang Master and Jiming Li Bachelor . Qingdao Shandong, China, Qingdao Central Blood Station, 266071, Central Laboratory .

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The subjects in this study were drawn from 1658 northern Han Chinese populations from the Chinese Bone Marrow Bank Registry in Qingdao, China. All samples were tested for HLA-A,B,DR by PCR-SSP using Pel-Freez SSP UNITRAY and One Lambda Micro SSPABDR kit. For HLA-A, most common antigens are A2(GF=0.2838), A24(GF=0.1521), A11(GF=0.1474), A30(GF=0.1235) and A33(GF=0.1043), rare phenotypes are A66(GF=0.0006) and A69(GF=0.0012). Prevalent HLA-B phenotypes are B13(GF=0.1521), B62(GF=0.0711), B61(GF=0.0635), B46(GF=0.0606) and B35(GF=0.0583). Lower frequencies of HLA-B phenotypes are B47(GF=0.0006), B81(GF=0.0006), B45(GF=0.0006), B70(GF=0.0006) and B42(GF=0.0006). DRB1*07, 65105;DRB1*15, DRB1*09 , DRB1*04 and DRB1*12 have been found with higher frequencies, respective GF is 0.1696, 0.1498, 0.1107, 0.1096 and 0.1037. DRB1*18 has lower frequency, its GF is 0.0006. The results of our investigation have similarities to other northern Han Chinese, besides the gene frequency of B*13 is remarkably higher than other northern Han Chinese results. By comparing with other regions and races, we can find that the distribution of HLA-A, B, DR gene frequency in northern Han Chinese population is quite different from that of southern Han Chinese, especially from that of other racial population. This indicates that it is very necessary to supplement more donors’ tissue typing data of different regions and races in China. There will be more patients who may acquire more opportunities to cure their fatal diseases by bone marrow transplantation.