6.2
#179
VARIABILITY OF HLA-B*27 ALLELES IN GERMAN PATIENTS SUSPECTED OF HAVING ANKYLOSING SPONDYLITIS:.
Alois Woelpl , Franziska Kastl , Alexandra Simon , Ingrid Lenggeler , Daniela Stauber and Hans-Georg Klein . Martinsried Germany, Laboratory for Medical Genetics, 82152, Dept. of Immunogenetics .

The distribution of B*27 alleles (B*2701-2725) was characterized on 2091 serologically typed patients living in Germany suspected of having ankylosis spondylitis or related spondyloarthropathies. The allelic typing was done by PCR-SSP high resolution. Testing with DNA methods 137 (6.5%) samples were B*27 negative. As documented in the following table 11 different B*27 alleles were identified. The genotypefrequence of the most common alleles in Germany B*2702 and B*2705 were comparable with the data published. 21 patients with low frequency allels were German origin, 13 coming from Turky or oriental countries. To confirm the results the rare subtypes are being sequenced.

Table 1: Genotypefrequence of HLA-B*27 Alleles
HLA-B27 SubtypesN=number of patientsgenotypefrequence
B*27022900.0777
B*270310.0003
B*270450.0013
B*270516300.5928
B*270780.0021
B*270960.0016
B*271020.0005
B*271210.0003
B*271310.0003
B*271490.0024
B*271910.0003